The IndiSpin Pathogen Kit is designed for high-quality and efficient extraction of pathogen nucleic acids, including viral RNA and DNA, and bacterial DNA. It is suitable for use with a wide variety of sample types such as whole blood, serum, plasma, other body fluids, swabs, washes, and tissue homogenates. Key features of the kit include:•A unified protocol for both DNA and RNA extraction.•Effective removal of inhibitors and contaminants for high-purity results.•Compatibility with automation on the QIAcube for low to medium throughput workflows.•A centrifugation-based protocol for straightforward implementation.The purified nucleic acids are free of proteins, nucleases, and other impurities, making them ideal for downstream applications, including real-time PCR-based pathogen detection. This versatile kit is particularly well-suited for applications in fields such as veterinary diagnostics, where sample types can vary significantly.